Journal: Journal of Virology

Article Title: Infection and Replication of Influenza Virus at the Ocular Surface

doi: 10.1128/JVI.02192-17

Figure Lengend Snippet: Influenza virus inhibition by human lactoferrin and lysozyme. Lyophilized human lactoferrin or lysozyme was resuspended in PBS at a concentration of 0.15 mg/ml and then mixed with an equal volume of virus. The mixture was incubated at room temperature for 1 h and then titrated via plaque assay. Binomial means of data from three experiments and 95% confidence intervals are shown. White bars, respiratory viruses; black bars, ocular viruses; gray bars indicate that NL/219 virus is unique among the ocular viruses.

Article Snippet: Lyophilized human milk lactoferrin (Athens Research and Technology) and recombinant human lysozyme (Sigma-Aldrich) were resuspended in PBS and used at a final concentration of 0.15 mg/ml, equivalent to that expected to be present in a 10-fold dilution of human tears ( 22 , 55 , – 58 ).

Techniques: Virus, Inhibition, Concentration Assay, Incubation, Plaque Assay

Journal: Acta diabetologica

Article Title: A rare missense variant in the milk fat globule-EGF factor 8 (MFGE8) increases T2DM susceptibility and cardiovascular disease risk with population-specific effects

doi: 10.1007/s00592-019-01463-x

Figure Lengend Snippet: a The distribution of circulating Mfge8 concentrations by MFGE8 genotypes. b The distribution of serum triglyceride levels by MFGE8 genotypes. p values are from the analysis of covariance (ANCOVA) showing difference between group means after adjusting for the confounding effects of age, gender, and BMI. Serum Mfge8 measures were available in 78 subjects including 39 T2DM cases and age- and gender-matched 39 controls

Article Snippet: Circulating concentrations of Mfge8 levels were quantified using frozen serum aliquots by enzyme-linked immunosorbent assay (ELISA) kits from Boster Biological Technology (Pleasanton, CA, USA) following the standard protocols following manufacturer’s instructions. ( www.bosterbio.com ).

Techniques:

Journal: Acta diabetologica

Article Title: A rare missense variant in the milk fat globule-EGF factor 8 (MFGE8) increases T2DM susceptibility and cardiovascular disease risk with population-specific effects

doi: 10.1007/s00592-019-01463-x

Figure Lengend Snippet: Multivariate regression analysis showing interactions between [T2DM status * Mfge8 concentrations] and [MFGE8 genotype * Mfge8 concentrations] for affecting triglyceride levels

Article Snippet: Circulating concentrations of Mfge8 levels were quantified using frozen serum aliquots by enzyme-linked immunosorbent assay (ELISA) kits from Boster Biological Technology (Pleasanton, CA, USA) following the standard protocols following manufacturer’s instructions. ( www.bosterbio.com ).

Techniques:

Journal: Acta diabetologica

Article Title: A rare missense variant in the milk fat globule-EGF factor 8 (MFGE8) increases T2DM susceptibility and cardiovascular disease risk with population-specific effects

doi: 10.1007/s00592-019-01463-x

Figure Lengend Snippet: Distribution of rs371227978 ( MFGE8 ) genotypes and allele frequencies in study samples stratified by diabetes status and association testing using logistic regression modeling

Article Snippet: Circulating concentrations of Mfge8 levels were quantified using frozen serum aliquots by enzyme-linked immunosorbent assay (ELISA) kits from Boster Biological Technology (Pleasanton, CA, USA) following the standard protocols following manufacturer’s instructions. ( www.bosterbio.com ).

Techniques:

Journal: Acta diabetologica

Article Title: A rare missense variant in the milk fat globule-EGF factor 8 (MFGE8) increases T2DM susceptibility and cardiovascular disease risk with population-specific effects

doi: 10.1007/s00592-019-01463-x

Figure Lengend Snippet: Multivariate linear regression analysis modeling showing genotype–phenotype association of MFGE8 variant for affecting serum Mfge8 concentrations

Article Snippet: Circulating concentrations of Mfge8 levels were quantified using frozen serum aliquots by enzyme-linked immunosorbent assay (ELISA) kits from Boster Biological Technology (Pleasanton, CA, USA) following the standard protocols following manufacturer’s instructions. ( www.bosterbio.com ).

Techniques: Variant Assay